Microscopic changes due to fumonisin B and ochratoxin A

Aim: The present study was designed to evaluate the toxic interaction of fumonisin B and ochratoxin A, alone and in 1 combination for pathology of the kidneys in day old Japanese quail. Materials and Methods: Day old Japanese quail chicks were divided into 4 groups and fed with basal quail chick mash containing fumonisin B @ 200 ppm (Group FX), Ochratoxin A @ 2 ppm (Group OX), and fumonisin B @ 200 ppm + 1 1 Ochratoxin A @ 2 ppm (Group FO) and standard toxin free feed (Group CX, control) for 28 days. Results: The quail chicks were assessed for various microscopic changes in the kidneys. Mean microscopic score values for congestion was found to be significantly higher (P< 0.05) in FO group when compared to that of other groups FX and OX at 7 and 21 DPF. Similarly, mean microscopic score values for degeneration was found to be significantly higher (P < 0.05) in FO group as compared to that of groups FX and OX at 7 and 14 DPF. Mean microscopic score values for necrosis and luminal hyaline bodies were significantly higher (P < 0.05) in FO group when compared to that of groups OX and FX at 7 DPF. Conclusion: Total score card for microscopic lesions observed in the kidneys when compared between different treatment groups showed that the lesions appeared to be more pronounced in the OTA fed group at 14 DPF as compared to other treatment groups. The overall lesion score values after the conclusion of the experiment were found to be higher in the combination group followed by the groups OX and FX, respectively.